LACTATIONAL EXPOSURE TO DI-2-ETHYL HEXYL PHTHALATE (DEHP) INDUCES OXIDATIVE STRESS AND CAUSES NEURODEGENERATION IN HIPPOCAMPUS OF OFFSPRING FEMALE ALBINO RATS

Authors

  • Balakrishnan Solaimuthu Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai - 600113.
  • Firdous Ahmad Bhat Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai - 600113.
  • Suganya Sekaran Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai - 600113
  • Mayakannan Manikandan Department of Genetics, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai - 600113.
  • Priyanka Balaganapathy Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai - 600113.
  • Janaki Gunasekharan Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai - 600113.
  • Arunakaran Jagadeesan Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai - 600113.

Keywords:

Phthalates, Lipid peroxidation, Superoxide dismutase, estrogen receptor, Bcl-2, Nissl’s granules

Abstract

The purpose of this study was to determine the effect of DEHP on the hippocampus of F1 progeny female albino rats. Healthy mother rats were divided into four groups. The first group (control) was orally fed with olive oil–vehicle and the other three groups were treated with DEHP 1, 10 and 100 mg /kg bw/day via oral gavage from PND (Post natal day) 1 till 21. On PND 60 the offspring female rats were sacrificed, their hippocampus dissected and processed for biochemical analysis. Results showed that DEHP induced oxidative stress in hippocampus by increasing reactive oxygen species and decreasing antioxidant enzymes. Further, DEHP reduced the mRNA expression of ER?, ? and decreased that of Bcl2. Construction of a protein-protein interaction map with ER?, ?, Bcl2 and their downstream interacting partners confirmed the existence of a network, enriched for molecular functions. To conclude, lactational exposure to DEHP causes hippocampal neurotoxicity. 

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Published

2014-06-30
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Balakrishnan Solaimuthu, Firdous Ahmad Bhat, Suganya Sekaran, Mayakannan Manikandan, Priyanka Balaganapathy, Janaki Gunasekharan, and Arunakaran Jagadeesan. “LACTATIONAL EXPOSURE TO DI-2-ETHYL HEXYL PHTHALATE (DEHP) INDUCES OXIDATIVE STRESS AND CAUSES NEURODEGENERATION IN HIPPOCAMPUS OF OFFSPRING FEMALE ALBINO RATS”. International Journal of Pharmaceutics and Drug Analysis, vol. 2, no. 6, June 2014, pp. 541-52, https://ijpda.com/index.php/journal/article/view/81.

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