DEVELOPMENT AND VALIDATION OF REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR ESTIMATION OF NICOTINE IN NICOTINE GUM TABLET
Keywords:Nicotine, RP-HPLC, Photo Diode Array, Method validation
This research manuscript describes simple, sensitive, accurate, precise and repeatable reverse phase high performance liquid chromatography method for the determination of nicotine in gum tablet. The sample was analyzed by reverse phase C18 column (Phenomenex C18, 250 mm × 4.6 mm, 5µ) as stationary phase; acetonitrile : phosphate buffer (PH-3.5) (70 : 30 , v/v) as a mobile phase at a flow rate of 1.0 ml/min. Quantification was achieved with Photo Diode Array detector at 260 nm. The retention time for nicotine was found to be 3.5 min. The linearity was obtained in the concentration range of 0.2-1.2 µg/ml for nicotine. The method was successfully applied to gum tablet dosage form because no chromatographic interferences from formulation excipients were found. The method retained its accuracy and precision when the standard addition technique was applied.
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