DEVELOPMENT AND VALIDATION OF REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR ESTIMATION OF NICOTINE IN NICOTINE GUM TABLET

Authors

  • PATEL JIGNESH V Department of Pharmaceutical Quality Assurance, Shree S. K. Patel College of Pharmaceutical Education & Research, Ganpat University, Kherva – 382711, Mehsana, Gujarat, India.
  • PATEL DIPTI B Department of Pharmaceutical Quality Assurance, Shree S. K. Patel College of Pharmaceutical Education & Research, Ganpat University, Kherva – 382711, Mehsana, Gujarat, India.

Keywords:

Nicotine, RP-HPLC, Photo Diode Array, Method validation

Abstract

This research manuscript describes simple, sensitive, accurate, precise and repeatable reverse phase high performance liquid chromatography method for the determination of nicotine in gum tablet. The sample was analyzed by reverse phase C18 column (Phenomenex C18, 250 mm × 4.6 mm, 5µ) as stationary phase; acetonitrile : phosphate buffer (PH-3.5) (70 : 30 , v/v) as a mobile phase at a flow rate of 1.0 ml/min. Quantification was achieved with Photo Diode Array detector at 260 nm. The retention time for nicotine was found to be 3.5 min. The linearity was obtained in the concentration range of 0.2-1.2 µg/ml for nicotine. The method was successfully applied to gum tablet dosage form because no chromatographic interferences from formulation excipients were found. The method retained its accuracy and precision when the standard addition technique was applied.

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References

Maryadele J O’ Neil ed, Merck Index, 14th Edition, Merck & Co. Inc, White House Station NJ: 1177,2005.

U. S. Pharmacopoeia/ NF Vol. III. The United states pharmacopeial convention, Twinbrook pathway Rockvillae, 3079,2009.

British Pharmacopoeia. Vol. ll. Stationary office. London Medicines and Healthcare product regulatory agency, 1501, 2010.

European Pharmacopoeia 6.0. Vol. II. 6th edition. Starboary: Council of Europe, 2500, 2007.

James A., Denise E. Quantitation of Major Tobacco Alkaloids by High-Performance Liquid Chromatography, Journal of Chromatography., 205: 147–154, 1981.

Laurian V., Lorena F., Ioana E. Determination of nicotine from tobacco by LC/MS/MS, Studia Universitais babebolyai, Physica, L., 4b: 19-24, 2005.

Lionel P., Christine P., Sophie L. Determination of nicotine in tobacco by HPTLC, ICH harmonized tripartile guidelines, validation of analytical procedures, text & methodology., Q2A(R1): 127-133, 2005.

Jin-ying1 S., Xiao-yu1 X., Huan Y., Tian-yan1 Y,. Determination of Nicotine in Tobacco by Capillary Electrophoresis with Electrochemical Detection, Chem. Res. Chinese Universities., 28(3): 415—418, 2012.

Lochmann H., Bazzanella A., Kropsch S.Determination of tobacco alkaloids in single plant cells by capillary electrophoresis, Journal of Chromatography., 917: 311–317, 2001.

Hiroyuki K., Reiko I., Katsuharu Y., Keita S. Determination of nicotine, cotinine, and related alkaloids in human urine and saliva by automated in-tube solid-phase microextraction coupled with liquid chromatography-mass spectrometry, J Pharm Biomed Anal., 625-635, 2008.

K.N Patel. Journal of Pharmacy Research, Derivative Spectrophotometric Method for Simultaneous Estimation of nicotine and Bupropion Hydrochloride in Synthetic Mixture., 2(9): 1525-1527, 2009

Laura J.L., John D.L., Delbert J.E., Lee D.H., Edwin A.L. Determination of Nicotine and Cotinine by Ion Pair Reversed-Phase Chromatography, Journal of Chromatographic Science. 28(4): 200-203, 1990.

Published

2014-04-15
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PATEL JIGNESH V, and PATEL DIPTI B. “DEVELOPMENT AND VALIDATION OF REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR ESTIMATION OF NICOTINE IN NICOTINE GUM TABLET”. International Journal of Pharmaceutics and Drug Analysis, vol. 2, no. 4, Apr. 2014, pp. 291-5, https://ijpda.com/index.php/journal/article/view/51.

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