RP-HPLC method development and validation for simultaneous estimation of efonidipine hydrochloride ethanolate and telmisartan in their synthetic mixture

Authors

  • Grishma H. Patel Pioneer Pharmacy Degree College, Ajwa-Nimeta road, Vadodara-390019, Gujarat, India
  • Shreya D. Adeshra Pioneer Pharmacy Degree College, Ajwa-Nimeta road, Vadodara-390019, Gujarat, India
  • Dhananjay B. Meshram Pioneer Pharmacy Degree College, Ajwa-Nimeta road, Vadodara-390019, Gujarat, India

DOI:

https://doi.org/10.47957/ijpda.v9i3.480

Keywords:

RP-HPLC, Efonidipine Hydrochloride Ehanolate and Telmisartan.

Abstract

A Novel, selective, accurate and rapid Reversed Phase High Performance Liquid Chromatographic (RPHPLC) method for the analysis of Efonidipine Hydrochloride Ethanolate and Telmisartan in binary mixture has been developed and validated. The chromatographic system consisted of a Phenomenex Kinetex ® 5µ C18 Size: 150 * 4.6mm column and the separation was achieved by using ambient temperature with a mobile phase containing mobile Phase Acetonitrile:25mM Phosphate Buffer pH 4.9 (45:55). The samples were monitored at 253 nm for detection at a flow rate of 1.0 mL/min and the retention time was about 7.77 and 4.10 mins for Efonidipine Hydrochloride Ehanolate and Telmisartan respectively. The calibration curve was linear over the concentration range 5-30 and 10-60 ?g/mL for Efonidipine Hydrochloride Ehanolate and Telmisartan respectively. The proposed method is accurate in the range of 99.75% - 100.10% recovery and precise (%RSD of intraday variation and % RSD of inter day variation were found to be within the acceptance criteria). Therefore, this method can be used as a more convenient and efficient option for the analysis of Efonidipine Hydrochloride Ehanolate and Telmisartan in Quality control laboratory.

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Published

2021-10-04
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How to Cite

patel, G. H., S. D. Adeshra, and D. B. Meshram. “RP-HPLC Method Development and Validation for Simultaneous Estimation of Efonidipine Hydrochloride Ethanolate and Telmisartan in Their Synthetic Mixture”. International Journal of Pharmaceutics and Drug Analysis, vol. 9, no. 3, Oct. 2021, pp. 190-5, doi:10.47957/ijpda.v9i3.480.

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