A validated stability indicating RP-HPLC method development and validation of for simultaneous estimation of indacaterol and glycopyrrolate in pharmaceutical dosage form


  • Vani Gajula Department of Pharmaceutical analysis, M.L.College of Pharmacy, S. Konda-523101.
  • Thejomoorthy K Head, Department of Pharmaceutical analysis, M.L.College of Pharmacy, S. Konda-523101.
  • Sreenivasa Prasanna P Principal, M.L.College of Pharmacy, S.Konda-523101




Indacaterol, Glycopyrronium, RP-HPLC


A simple, Accurate, precise method was developed for the simultaneous estimation of the Indacaterol and Glycopyrronium in the bulk and pharmaceutical dosage form. The chromatogram was run through Std Denali C18150 x 4.6 mm, 5m. Mobile phase containing Buffer 0.1%OPA: Acetonitrile taken in the ratio 55:45 was pumped through the column at a flow rate of 0.8 ml/min. The buffer used in this method was 0.1% OPA buffer. The temperature was maintained at 30°C. The optimized wavelength selected was 230.0 nm. The retention time of Indacaterol and Glycopyrronium were found to 2.323min and 3.140 %RSD of the Indacaterol and Glycopyrronium were %RSD found to be 0.2% and 0.2% respectively. %Recovery was obtained as 99.39% and 99.41% for Indacaterol and Glycopyrronium respectively. LOD, LOQ values obtained from regression equations of Indacaterol and Glycopyrronium were 1.08, 3.28, and 0.25, 1.47 respectively. Regression equation of Indacaterol is y = 24501x + 7142.3, y = 26335x + 7822.7of Glycopyrronium.Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control tests in Industries.


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How to Cite

Gajula , V., T. K, and S. P. P. “A Validated Stability Indicating RP-HPLC Method Development and Validation of for Simultaneous Estimation of Indacaterol and Glycopyrrolate in Pharmaceutical Dosage Form”. International Journal of Pharmaceutics and Drug Analysis, vol. 9, no. 2, July 2021, pp. 71-79, doi:10.47957/ijpda.v9i2.464.



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