Novel method development and validation rp-hplc for simultaneous determination of darunavir and cobicistat in bulk and pharmaceutical formulation

Authors

  • Kumaraswamy.Gandla Care College of Pharmacy, Oglapur (Vill),Damera,(Mdl),Warangal-Rural,(Dist)-506001.Telangana, India.
  • R.Lalitha Chaitanya College of Pharmacy Education and Research, Hanamkonda, Warangal, Telangana 506001
  • M.Murali Krishna Research Scholar, Career Point University, Kota, Rajasthan,-324005,India
  • P.VR Teja Shruthi Research Scholar, Career Point University, Kota, Rajasthan,-324005,India

Keywords:

RP-HPLC, Darunavir, Cobicistat, ART

Abstract

A stability indicating reverse phase High performance liquid chromatography (RP-HPLC) method has been developed and subsequently validated for the simultaneous determination of Darunavir and Cobicistat in bulk and pharmaceutical formulation. Separation was achieved in isocratic mode with a Kinetex C18 100 A (250 mm x 4.6 mm, 5?) column and mixture consisting of 0.1% OPA(pH 3) and methanol in 80:20 v/v was used as mobile phase with a flow rate of 1 ml/min, column temperature at 25°C and the run time as 10 mins. UV detection was performed at 239 nm and the sample temperature was maintained ambient. The described method for simultaneous determination of Darunavir and Cobicistat is linear over a range of 8 ?g/ml to 120 ?g/ml and 5 ?g/ml to 60 ?g/ml respectively. The method shows good precision results which were below 2.0%RSD. Limit of Detection (LOD) and Limit of Quantification (LOQ) of Darunavir and Cobicistat was established and found to be 1.49 and 4.97 ?g/ml and 1.13 and 3.77 ?g/ ml respectively. The developed method was validated according to ICH guidelines for various parameters. The method is simple, rapid, selective and stability indicating method which would be used for regular stability indicating quality control determinations.

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References

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Published

2020-02-29
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How to Cite

Kumaraswamy.Gandla, R.Lalitha, M.Murali Krishna, and P.VR Teja Shruthi. “Novel Method Development and Validation Rp-Hplc for Simultaneous Determination of Darunavir and Cobicistat in Bulk and Pharmaceutical Formulation”. International Journal of Pharmaceutics and Drug Analysis, vol. 8, no. 2, Feb. 2020, pp. 6-9, https://ijpda.com/index.php/journal/article/view/460.

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