Study of Antimicrobial and Antioxidant potentiality of Anti-diabetic drug Metformin


  • Fatema Nasrin Department of Pharmacy, Southeast University, Banani, Dhaka-1213, Bangladesh.


Metformin, Antimicrobial activity, zone of inhibition, antioxidant, free radical


Metformin, an oral hypoglycemic drug was studied in vitro for possible antibacterial and antioxidant activity. Antimicrobial activity of metformin was evaluated against 9 Gram-positive, Gram-negative bacteria and 3 fungi using disk diffusion technique. Kanamycin (30?g/disc) was used as standard. Antioxidant potentiality of metformin was investigated by DPPH scavenging activity. Metformin active at 500 ?g/ml, showed very good antimicrobial activity against most of the bacterial strains with an average zone of inhibition of 12-15mm. Metformin demonstrated a pronounced inhibitory action against Pseudomonas aeruginosa, an organism which is known to be multidrug resistant. The tested fungi are Saccharromyces cerevaceae, Candida albicans and Aspergillus niger. The tested drug showed very good antifungal activity with an average 13 -17 mm zone of inhibition. The percentage (%) scavenging of DPPH free radical of metformin was found to be concentration dependent with IC50 value 56.90± 0.83?g ?g/ml while IC50 value of standard ascorbic acid was found to be 51.89 ± 1.11?g/ml. The results of these studies indicated that metformin could be significantly protect animals from tested pathogenic bacteria as well as might be beneficial for diabetic patient more than a hypoglycemic drug.


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Berger-Bächi B. Resistance mechanisms of grampositive

bacteria. International Journal of Medicine

& Microbiology. 2002; 292:27–35.

Jette E. Kristiansen,Oliver Hendricks, Thomas

Delvin, Trine S. Butterworth, Lone Aagaard, Jørn B.

Christensen, Vivian C. Flores and Hendrik Keyzer.

Reversal of resistance in microorganisms by help of

non-antibiotics. Journal of Antimicrobial

Chemotherapy.2007; 59 ( 6 ) : 1271-1279.

Kristiansen, J. E. Antimicrobial activity of

nonantibiotics.ASM NEWS.1991; 57:135–139.

Kristiansen, J. E. Antimicrobial activity of

nonantibiotics. Reports from a congress on the

antimicrobial effect of drugs other than antibiotics

on bacteria, viruses, protozoa, and other organisms.

Copenhagen, May, 1990. Acta Pathology

Microbiology et Immunology Scandinavia

(APMIS), Suppl. 1992 ;100 :7–14.

Cederlund, H. & Mårdh, P. A. Antibacterial

activities ofnon-antibiotic drugs. Journal of

Antimicrobial Chemotherapy. 1993; 32:355–365.

Kristiansen, J. E. H. Chlorpromazine: non-antibiotics

with antimicrobial activity—new insights in

managing resistance. Current Opinion in

Investigational Drugs.1993; 2: 587–591.

Kristiansen, J. E., Amaral, L. & Thomsen, V. F. The

reversal of antibiotic resistance by phenothiazines.

In Abstract 18th International Congress of

Chemotherapy. Stockholm, Sweden. 1993. Abstract

, p. 65.

Amaral, L., Kristiansen, J. E. & Thomsen, V. F. The

effect of chlorpromazine on the cell envelope of

sensitive and resistant bacteria. In Abstract of the

th International Congress of Chemotherapy.

Stockholm, Sweden. 1993, Abstract 493 p. 65.

Dastidar SG, Saha PK, Sanyamat B, Chakrabarty

AN. Antibacterial activities of Ambodryl and

Benadryl. Journal of Applied Bacteria. 1976; 41:


Chakrabarty AN, Acharya DP, Niyogi DK, Dastidar

SG. Drug interaction of some non-conventional

antimicrobial chemotherapeutic agents with special

reference to Promethazine. Indian Journal of

Medicine & Research. 1989; 89: 233-237

Dash SK,DastidarSG, Chakrabarty AN.Antimicrobial

activity of Promazine Hydrochloride. Indian Journal

of Experimental Biology. 1977; 15: 324-326.

Molnár J, Mandi Y, Király J. Antibacterial effect of

some phenothiazine compounds and R-factor

elimination by chlorpromazine. Acta Microbiology.

Acad Sci Hung 1976; 23: 45-54.

Dastidar SG, Mondal U, Niyogi S, Chakrabarty AN.

Antibacterial property of methyl-DOPA and

Development of cross-resistance in m-DOPA

mutants. Indian Journal of Medicine & Research

.1986; 84:142-147.

Chakrabarty AN, Molnár J, Dastidar SG, Motohashi

N.(Eds) Non-antibiotics: A new class of

unrecognised antimicrobics: National Institute of

Science Communication, New Delhi. 1998.


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How to Cite

Fatema Nasrin. “Study of Antimicrobial and Antioxidant Potentiality of Anti-Diabetic Drug Metformin”. International Journal of Pharmaceutics and Drug Analysis, vol. 2, no. 3, Mar. 2014, pp. 220-4,



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