EFFECTS OF MISHENLAND ANTI-DIABETIC HERB ON THE KIDNEY OF STREPTOZOTOCIN-INDUCED DIABETIC ADULT WISTAR RATS
Keywords:
Diabetes mellitus, streptozotocin, misheland herb, LDH, G6PDH, wistar ratsAbstract
This study investigates some of the effects of Mischeland anti-diabetic herb in the kidney of diabetic adult wistar rats. Mishenland is an herbal mixture consisting of alkaloids, anthraquinone, cylogenetic glycosides, saponin and tannins. Diabetes is a serious lifelong multi-system disorder of glucose metabolism in the body. It is a condition characterized by high blood sugar level that results from defect in insulin secretion, or action, or both. 36 adult wistar rats of both sexes weighing between 195g – 280g were randomly divided into three groups; A, B and C. Diabetes mellitus was induced in group B and C rats by single intraperitoneal injection of streptozotocin (STZ) at a dosage of 70mg/Kg b.w, freshly dissolved in 1ml citrate buffer solution at pH of 4.5. The control rats (group A) were injected with only citrate buffer solution intraperitoneally. At the beginning of the 7th week post STZ injection, mischeland anti-diabetic herb was administered at a dosage of 0.92g/Kg b.w for rats in group C with the diabetic group B rats left untreated. The animals in group C were further subdivided into C1, C2 and C3. Group C1 received a normal dose of 0.92g/Kg b.w, C2 animals were administered additional 100% of C1 dosage (serving as high dose) and group C3 received 50% of group C1 dosage which served as low dose. This mishenland anti-diabetic herb treatment lasted for another six
weeks, post STZ induction. The result shows that the mean body weight of diabetic group (Group B) was significantly reduced compared tothe control group A. It was also observed that the treated groups (C1, C2 and C3) regained a significant amount of body weight, following treatment with mishenland herb. In addition, it was also observed that the increased glucose level (in
diabetic group B rats) was significantly reversed in the mishenland treated group C rats. G6PDH and LDH levels in the kidney and blood of treated rats were significantly increased, compared to their low levels in diabetic group B rats. This experimental work reveals mishenland anti-diabetic herb as a promising therapy for the reversal of the dreadful conditions caused by diabetes if further researched up on. All results were expressed as Mean ± Standard Deviation (S.D) for each group. All grouped data were statistically evaluated using SPSS 15.0 software. Hypothesis testing methods included the independent – samples t–test. Statistical
significance was set at p<0.05.
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