Studies On In Vitro Anti-Microbial Activity By Disc Diffusion Method And FTIR Analysis OF Achyranthes aspera (L.)


  • Thafshila Aafrin AM PG and Research Department of Biochemistry, Sengamala Thayaar Educational Trust Women’s College, Sundarakkottai, Mannargudi-614 016
  • Anuradha, R PG and Research Department of Biochemistry, Sengamala Thayaar Educational Trust Women’s College, Sundarakkottai, Mannargudi-614 016.


Antimicrobial activity, Achyranthes aspera, FTIR spectral analysis.


The antimicrobial activity of Achyranthes aspera against the bacteria and fungi plays a vital role in evaluation of the ethanomedicinal property of plants. FT-IR spectral analysis was carried out in ethanolic extract. The FT-IR analysis has revealed the presence of alcohols, alkanes, terminal alkyne, alkenes, halogen, ethers, aromatic compounds, aliphatic chloro compounds and aryldisulphides respectively. This provides and supports the therapeutical information of the plants. The medicinal values of Achyranthes aspera were investigated through agar well disc diffusion method. The aqueous and ethanolic extracts of plants to be tested were prepared at a concentration of 200µg/mL were dissolved in dimethyl sulphoxide (Dimethyl Sulphoxide (DMSO).The zone of inhibition gives the degree antimicrobial property. It was compared with standard antimicrobial agents like Gentamycin for bacteria and Amphotericin for fungi. From this study, it can be concluded that Achyranthes aspera exhibits antimicrobial activity against certain microorganisms.


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Thafshila Aafrin AM, and Anuradha, R. “Studies On In Vitro Anti-Microbial Activity By Disc Diffusion Method And FTIR Analysis OF Achyranthes Aspera (L.) ”. International Journal of Pharmaceutics and Drug Analysis, vol. 6, no. 4, Apr. 2018, pp. 446-50,



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