Qualitative And Quantitative Estimation Of Gallic Acid In Rohitakarishta Formulation


  • Vaishali Argade Department of Quality Assurance Techniques (PG), Pravara Rural Education Society’s, Institute of Pharmacy LoniKd.,Dist-Ahemadnagar, Maharashtra, India
  • Yogesh Thombare Department of Quality Assurance Techniques (PG), Pravara Rural Education Society’s, Institute of Pharmacy LoniKd.,Dist-Ahemadnagar, Maharashtra, India


Rohitakarishta, FT-IR, HPTLC, UV method development


Rohitakarishta is one of the Ayurvedic polyherbal formulation mentions in Ayurvedic Pharmacopoeias, which protect against anemia, spleen and liver disorders. The qualitative estimation of Gallic acid from Rohitakariasta formulation was done by using IR, HPTLC techniques and result stated the significant presence of Gallic acid in Rohitakarishta formulation.  The suitability of UV method for quantitative determination was proved by validation as per ICH Q2B guidelines.The quality assessment of Rohitakarishta for total microbial content was also analyzed as per WHO guidelines and shows greater therapeutic efficacy.


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B. N. Nandre, S.R. Bakliwal, B. R. Rane, S. P. Pawar, Traditional Fermented Formulation- Asava and Arishta, International Journal of Pharmaceutical and Biological Archives 2012: 3(6):1313-1319.

Vandana V. Kadlag, Veena S. Kasture, Seema A. Gosavi, and Rasika D. Bhalke. Standardization of mar-keted Adulsa syrup containing Vasaka by High performance thin layer Chromatography. Asian Journal of Chemistry 2011:23(5): 1917-1921.

S Sekar and S Mariappam. Traditional fermented biomedicines Arishtas&Asava from Ayurveda. Indian Journal of Traditional Knowledge 2008: 7(4): 548-556.

S. F. Sayyad, D. S. Randive, S.M. Jagtap, S. R. Chaudhari and B. P. Panda, Preparation and evaluation of fermented Ayurvedic Formulation, Journal of Applied Pharmaceutical Science 2012:2(5):122-124.

M. ObayedUllah, Kaiser Hamid, KAshfaqur Rahman, MSK Chaudhari, Effect of Rohitakarishta; an Ay-urvedic formulation on the lipid profile of rat plasma after chronic administration , Biology and medicine 2010, 2(2):26-31.

Dr. C. K. Kokate, A. P. Purohit, S. B. Gokhale.Pharmacognosy, 29th edition, New Delhi: Niraliprakashan 2002: 9-550.

The Ayurvedic Pharmacopoeia of India, Government of India, Ministry of Health and Family Welfare, Part II (Formulary), volume II, First edition, New Delhi, 2008: 58.

Dr. V D Rangari. Pharmacognosy and Phytochemistry. Volume I, 3rd edition, Carrier publication 6-10.

Manohar A Potdar. Pharmaceutical Quality Assurance. Second edition. NiraliPrakashan: 1.1-1.10.

A. Finose and K. Devaki, Phytochemical and chromatographic studies in the flowers of Woodfordiafruticosa (L) Kurz, Asian journal of plant science and research, 1(3) (2011) 81-85.

Varunkumar Singh, C R C Reddy. Validation of lodhradikashaya (Ayurvedic formulation) made by traditional method and contemporary spray drier methods through FTIR spectroscopy. International journal of pharmacy and analytical research 2015:4(3):386-390.

M. S. Kondawar, K. G. Kamble, D.S. Mali. Quantitative estimation of Gallic Acid and Ascorbis Acid in marketed herbal medicine TriphalaChurna by High Performance Thin layer chromatography. International Journal of PharmaTech Research 2011:3(3):1593-1599.

SiladityaBehera, SubhajitGhanty, Fahad Ahmad, saayakSantra, Sritoma Banerjee. UV Visible Spectrophotometric method developmentand Validation of Assay of Paracetamol Tablet Formulation. Journal of Analytical and Boianalytical Techniques2012:3:6.

Shreyash S Mutha, Veena S. Kasture, seema A. Gosavi, Rasika d bhalke, Sarita S. Pawar, Standardization of Abhyarishta as per WHO guidelines 2013:3(1):510-518.

Pavia D. L, Lampman G M, Kriz G. S, Vyan J R. Spectroscopy. Indian edition. Cengage Learning India Private Limited New Delhi 2007:409-410.

Skoog D A, Holler F J, Crouch S. R. Instrumental analysis. Indian edition, Cengage Learning India Private Limited New Delhi 2007:292-619.


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How to Cite

Vaishali Argade, and Yogesh Thombare. “Qualitative And Quantitative Estimation Of Gallic Acid In Rohitakarishta Formulation”. International Journal of Pharmaceutics and Drug Analysis, vol. 6, no. 3, Mar. 2018, pp. 377-83, https://ijpda.com/index.php/journal/article/view/388.



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