Development And Validation Of HPLC Method For The Determination Of Suvorexant In Pharmaceutical Dosage Forms

Authors

  • Sriram Siddhartha Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India.
  • Tata Santosh Pharmaceutical Research & Development Laboratory, Corpuscle Research Solutions, Visakhapatnam, India
  • J Vijaya Ratna Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India.

Keywords:

Suvorexant; Insomnia; Neurokinin receptor; HPLC, MK-4305, Orexin Receptor

Abstract

The present experiment was focused in developing an accurate and precise method for the estimation of Suvorexant in pharmaceutical Tablet dosage form. The mobile phase consisted of 65:35 % (v/v) of Methanol & 0.1% orthophosphoric acid solution operated on isocratic mode. The flow rate is 1.0 ml/min. Chromatographic determination of Suvorexant was performed on Agilent Zorbax 300oA Extend-C18 column (150 X 4.6 mm id, 5µm). The wavelength of detection is 248 nm. The injection volume is 20µL. The retention time of Suvorexant is 5.50 ± 0.10 minutes. The method was validated as per ICH guidelines and has been successfully used for the estimation in tablet dosage forms.

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References

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Published

2018-03-26
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How to Cite

Sriram Siddhartha, Tata Santosh, and J Vijaya Ratna. “Development And Validation Of HPLC Method For The Determination Of Suvorexant In Pharmaceutical Dosage Forms”. International Journal of Pharmaceutics and Drug Analysis, vol. 6, no. 3, Mar. 2018, pp. 425-34, https://ijpda.com/index.php/journal/article/view/385.

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