Antibacterial, Cytotoxic and Antioxidant Activities of n-Hexane, Chloroform and Ethyl Acetate extracts of Cucumis sativus leaves.


  • Israt Jahan Bulbul Assistant Professor, Department of Pharmacy, Southeast University, Banani, Dhaka-1213


C. sativus, antibacterial, antioxidant, cytotoxic


The main aim of this study was to find out the antibacterial, cytotoxic and antioxidant activities of n-hexane, chloroform and ethyl acetate extracts of C. sativus (Cucurbitaceae). Disc diffusion technique was used for in vitro anbacterial screening against gram positive, gram negative human pathogenic bacteria. Here kanamycin disc (30 mg/disc) was used as standard. The chloroform and the n-hexane extract of C. sativus showed moderate antibacterial activity with the average zone of inhibition 7-12 mm and 7-9 mm respectively. The brine shrimp lethality bioassay method was used to determine the cytotoxic activity and vincristine sulphate was used as positive control. Among the extractives the chloroform soluble fraction demonstrated the highest cytotoxic activity with LC50 16.89 µg/ml which indicates the compounds present in the chloroform extract are promisingly cytotoxic. Antioxidant activity test of the crude extracts were assessed by means of DPPH free radical scavenging method where ascorbic acid was used as standard. The chloroform fraction of C. sativus showed strongest antioxidant activity with IC50 value of 54.06 µg/ml. In case of phenolic content, the n-hexane, chloroform and ethyl acetate extracts of C. sativus revealed 16.08 mg/g, 19.04 mg/g and 46.01 mg/g of extractives, respectively.


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How to Cite

Israt Jahan Bulbul. “Antibacterial, Cytotoxic and Antioxidant Activities of N-Hexane, Chloroform and Ethyl Acetate Extracts of Cucumis Sativus Leaves.”. International Journal of Pharmaceutics and Drug Analysis, vol. 2, no. 3, Apr. 2014, pp. 280-4,



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