Dual Wavelength Spectrophotometric Method for Simultaneous Estimation of Ciprofloxacin and Phenylephrine Hydrochloride in Combined Dosage Form
Keywords:Ciprofloxacin, Phenylephrine Hydrochloride, Dual wavelength, Linearity, Validation
A simple, accurate and precise dual wavelength spectrophotometric method was developed for simultaneous determination of Ciprofloxacin and Phenylephrine Hydrochloride in combined pharmaceutical dosage form. The principle for dual wavelength method is “the absorbance difference between two points on the mixture spectra is directly proportional to the concentration of the component of interest”. The wavelengths selected for determination of Ciprofloxacin were 272.09 nm and 305.85 nm, whereas, the wavelengths selected for determination of Phenylephrine Hydrochloride were 256.80 nm and 282.96 nm. 0.1 M NaOH was taken as a solvent. Regression analysis of Beer’s plots showed good correlation in concentration range of 1?12 ?g/ml for Ciprofloxacin and 5-30 ?g/ml for Phenylephrine Hydrochloride. Accuracy of method was found between 99.93 ? 101.65 %. The precision (intra?day, inter?day and repeatability) of method was found within limits (%CV<2). The proposed methods are simple, rapid, economic and accurate for routine simultaneous estimation of Ciprofloxacin and Phenylephrine Hydrochloride.
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