Development and Validation of RP-HPLC Method For Simultaneous De-termination Of Rosuvastatin And Clopidogrel In Tablet Dosage Form
Keywords:RP HPLC, Rosuvastatin calcium, Clopidogrel bisulphate, Simultanious estimation, validation
The aimed of research the method development and validation by RP-HPLC method of the Rosuvastatin calcium and Clopidogrel bisulphate. The method is a simple, accurate, specific, precise, reproducible and sensitive. The ? max of ROSU and CLOP was found to be 240nm. Coefficient correlation 0.999, Beer’s Law limit 50-150 µg/ml, from the four trial of different concentration of mobile phase was selected Methanol:Water 80:20 v/v, pH 3.0 at 240nm, flow rate 1ml/min, sample inlet 20 µL, C 18 Prontosil, %RSD of ROSU 1.017 and CLOP 0.173, theoretical plates ROSU 7797.53 and ROSU 8257.53, Retention time ROSU 3.483min and CLOP 4.983min, Tailing factor ROSU 1.1787 and CLOP 1.074, limits 2 NMT, Accuracy ROSU 0.37 %RSD, Recovery 99.59% and CLOP 0.18 %RSD, recovery 100.41% was show good efficacy and results. The methods indicate Future scope in analysis quality control of the estimation of ROSU and CLOP for routine drug quality analysis investigation.
Solanki C, Patel N. Development and validation of RP-HPLC method for simultaneous estimation of rosuvastatin calcium and aspirin in capsule dosage form. Int J Pharm Bio Sci. 2012;3(3): 577-585.
Telrandhe R. Nanotechnology for cancer therapy: Recent developments. Eur J Pharm Med Res. 2016;3(11): 284-294.
Masud A A, Begum I. Development and validation of a RP HPLC method for simultaneous estimation of aspirine and clopidogrel in combined tablet dosage form. Int J Pharm Sci Res. 2016;7(11): 4443-4448.
Telrandhe R, Mahapatra D K, Kamble M A. Bombax ceiba thorn extract mediated synthesis of silver nanoparticles: Evaluation of anti-Staphylococcus aures activity. Int J Pharm Drug Anal. 2017;5(9): 376-379.
Purkar A J, Balap A R, Jadhav S B, Chaudhari P D. Development and validation of UV Spec-trophotometric method for simultaneous determination of rosuvastatin calcium and aspirin in its pure and pharmaceutical dosage forms. Int J Pharm Chem Sci. 2012;1(3): 1008-1012.
Donthula S, Kumar M K, Teja G S, Kumar Y M, Krishna J Y, Ramesh D. A new validated RP-HPLC method for determination of Rosuvastatin calcium in bulk and pharmaceutical dosage form. Der Pharmacia Lettre. 2011;3(3): 350-356.
Shende V, Telrandhe R. Formulation and evaluation of Tooth Gel from Aloe vera leaves extract. Int J Pharm Drug Anal. 2017;5(10): 394-398.
Yusuf S M, Samparna S, Yallareddy K, Pavani B, Sivakala T. Analytical method development and validation of atorvastatin and clopidogrel in tablet dosage form by RP-HPLC. Eur J Pharm Med Res. 2017;4(4): 553-558.
Deshmukh P, Telrandhe R, Gunde M. Formulation and Evalua-tion of Herbal Toothpaste: Compared With Maeketed Preparation. Int J Pharm Drug Anal. 2017;5(10): 406-410.
Trivedi L, Telrandhe R, Dhabarde D. Differential spectrophotometric method for estimation and validation of Verapamil in Tablet dosage form. Int J Pharm Drug Anal. 2017;5(11): 419-422.
Doshi N, Sheth A, Sharma A, Dave J B, Patel C N. Validated RP-HPLC method for simultaneous estima-tion of Rosuvastatin calcium and Telmisartan in pharmaceutical dosage form. J Chem Pharm Res. 2010;2(2): 252-263.
Chitlange S S, Shinde P S, Pawbake G R, Wankhede S B. Simultaneous estimation of thiocolchicoside and aceclofenac in pharmaceutical dosage form by spectrometric and LC method. Der Pharmacia Lettre. 2010;2(2): 86-93.
Mohammed I B, Karanam V P, Ghanta K M. RP-HPLC method for simultaneous estimation of rosuvastatin and ezetimibe from their combination tablet dosage form. Int J Chem Anal Sci. 2013;4: 205-209.
ICH. Proceedings of the Inrernational Conference on Harmonisation of Technical Requirement of Registration of Pharmaceuticals for Human Use (ICH Harmonised Tripartite Guidelines). Validation of Analytical Procedures: Methodology, Q2B; Geneva, Switzerland.
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