Development and Validation of RP-HPLC Method For Simultaneous De-termination Of Rosuvastatin And Clopidogrel In Tablet Dosage Form

Authors

  • Harshana Rothekar Department of Pharmaceutical chemistry, Siddhivinayak College of pharmacy, Warora, Maharashtra, India.
  • Roshan Telrandhe Kamla Nehru college of pharmacy, Butibori, Nagpur 441108, Maharashtra, India.
  • Prasad Jumade Department of pharmaceutical chemistry, Agnihotri College of pharmacy, Wardha, Maharashtra, India

Keywords:

RP HPLC, Rosuvastatin calcium, Clopidogrel bisulphate, Simultanious estimation, validation

Abstract

The aimed of research the method development and validation by RP-HPLC method of the Rosuvastatin calcium and Clopidogrel bisulphate. The method is a simple, accurate, specific, precise, reproducible and sensitive. The ? max of ROSU and CLOP was found to be 240nm. Coefficient correlation 0.999, Beer’s Law limit 50-150 µg/ml, from the four trial of different concentration of mobile phase was selected Methanol:Water 80:20 v/v, pH 3.0 at 240nm, flow rate 1ml/min, sample inlet 20 µL, C 18 Prontosil, %RSD of ROSU 1.017 and CLOP 0.173, theoretical plates ROSU 7797.53 and ROSU 8257.53, Retention time ROSU 3.483min and CLOP 4.983min, Tailing factor ROSU 1.1787 and CLOP 1.074, limits 2 NMT, Accuracy ROSU 0.37 %RSD, Recovery 99.59% and CLOP 0.18 %RSD, recovery 100.41% was show good efficacy and results. The methods indicate Future scope in analysis quality control of the estimation of ROSU and CLOP for routine drug quality analysis investigation.

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References

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Published

2017-12-13
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Harshana Rothekar, Roshan Telrandhe, and Prasad Jumade. “Development and Validation of RP-HPLC Method For Simultaneous De-Termination Of Rosuvastatin And Clopidogrel In Tablet Dosage Form”. International Journal of Pharmaceutics and Drug Analysis, Dec. 2017, pp. 475-82, https://ijpda.com/index.php/journal/article/view/321.

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