HPLC-UV Method for the determination of Biapenem in Pharmaceutical Dosage Forms
Keywords:
Biapenem; Antibiotics; RP- HPLCAbstract
A simple, selective, rapid, precise and economical reverse phase high pressure liquid chromatographic method has been developed for the estimation of Biapenem in pharmaceutical Tablet dosage form. The mobile phase consisted of 50:50 % (v/v) of Methanol & 10mM Potassium dihydrogen phosphate operated on isocratic mode. The flow rate is 1.0 ml/min. Chromatographic determination of Biapenem was performed on Agilent Zorbax SB C18 column (150 X 4.6 mm id, ODS 2, 5µm). The wavelength of detection is 300 nm. The injection volume is 20µL. The retention time of Biapenem is 2.72 ± 0.10 minutes. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation and solution stability. The influence of Acid, Alkaline, Oxidative Stress, Photolytic stress conditions on Biapenem was studied. Results indicated complete degradation in Alkaline medium. The proposed method has been successfully used for the estimation in dosage forms.
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