Development and Validation of UV Spectrophotometric Method for the Estimation of Curcumin in an Ayurvedic Formulation Haridrakhand
Keywords:
Haridrakhand, polyherbal formulation, Curcumin, UV spectrophotometry, Validation, ICHAbstract
A rapid, simple, selective and precise UV-Visible spectrophotometric method has been developed for the determination of curcumin in ayurvedic polyherbal formulation Haridrakhand. The intake formulation was subjected for maceration process using methanol as a solvent for the extraction of curcumin as a one of the ingredients in turmeric powder. The formulation containing 32 parts of turmeric powder out of total content. The spectrophotometric detection of curcumin was carried out at an absorption maximum of 421nm using methanol as a solvent. The method was validated according to ICH guidelines. The linearity range was found to be 1-25?g/ml with a correlation coefficient of 0.993. The accuracy was found to be within limit. The LOD and LOQ were found to be 1.29 and 4.3 ?g/ml; respectively. The results demonstrated that the method can be conveniently employed for routine quality control analysis of Curcumin in Ayurvedic formulation of Haridrakhand.
Downloads
References
Chaudhari Varsha , et al, a preliminary pharmacognostical and physicochemical assay of haridrakhanda granules: a pilot studyijrap 2011, 2 (6) 1681-1684 ,International Journal of Research in Ayurveda & Pharmacy ISSN 2229-3566 (Research Article)
V d. Joshi Y. G. editor, kayachikitsa 1st ed. Pune Balmitra Prakashan 2002 p.951, 952, 1010.
Bagchi A: Extraction of Curcumin. Journal of Environmental Science- Toxicology and Food Technology 2012; 1(3): 01-16.
Choudhary N and Singh B: Potential Therapeutic Effect of Curcumin - an update. Journal of Pharmaceutical Education Research 2012; 3(2): 64-71
H.H. Tonnesen, J.Z. Karlsen, Lebensm. Unters. Forsch., 1985, 180, 402-404.
G.W. Schieffer, J. Liq. Chromatogr. Related Technol., 2002, 25, 3033-3044.
M. Mahkam, M. Nabati, H.R. Kafshboran, Iran. Chem. Commun., 2014, 2, 172-178.
Srinivasan KR. A chromatographic study of the curcuminoids in curcuma longa Linn. J. Pharm. Pharmacol. 1953; 5: 448-453.
Marsin SM, Ahmad UK, Smith RM. Application of supercritical fluid extraction and chromatography to the analysis of turmeric. J Chromatogr Sc. 1993; 31: 20-25. 5
Jansirani.D1*, Saradha.R1, Salomideborani.N1, Selvapriyadharshini , Comparative evaluation of various extraction methods of curcuminoids from Curcuma longa. Journal of Chemical and Pharmaceutical SciencesISSN: 0974-2115
ICH Guideline Q2 (R1), Validation of analytical procedures: text and methodology, November 2005.
.ICH Harmonized Tripartite Guidelines, Validation of analytical procedures: text & bcmethodology, Q2 (R), Nov 2005
. Kunnumakkara AB, Anand P, Aggarwal BB: Curcumin inhibits proliferation, invasion, angiogenesis and metastasis of different cancers through interaction with multiple cell signaling proteins. Cancer Lett 2008; 269: 199–225.
Rubesh Kumar S, Ram kishan J, Venkateshwar Roa KN, Duganath N, R Kumanam. Simultaneous Spectrophotometric estimation of curcuminoid and gallic acid in bulk drug and ayurvedic polyherbal dosage forms, Journal of Chromatography B, 2003; 783 :287-295.
World Health Organization, Quality Control Methods for Medical Plants Materials, Geneva, 1998; 1-15. [updated 1998; cited 2011 Jul 9]. Available from: http://apps.who.int/medicinedocs/en/d/Js2200e/.
Pharmacoepical standards for Ayurvedic Formulations, Central Council for Research in Ayurvedic and Siddha, New Delhi: Ministry of Health and Family Welfare; 1987, 112-23.
Aarunava Gandait et al., Validated method for estimation of curcumin from turmeric powder.Ind.j.of Trad. Knowledge, 2011; 10(2): 247-250.
Published
How to Cite
Issue
Section

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright © Author(s) retain the copyright of this article.