Development and Validation of UV Spectrophotometric Method for the Estimation of Curcumin in an Ayurvedic Formulation Haridrakhand
Keywords:Haridrakhand, polyherbal formulation, Curcumin, UV spectrophotometry, Validation, ICH
A rapid, simple, selective and precise UV-Visible spectrophotometric method has been developed for the determination of curcumin in ayurvedic polyherbal formulation Haridrakhand. The intake formulation was subjected for maceration process using methanol as a solvent for the extraction of curcumin as a one of the ingredients in turmeric powder. The formulation containing 32 parts of turmeric powder out of total content. The spectrophotometric detection of curcumin was carried out at an absorption maximum of 421nm using methanol as a solvent. The method was validated according to ICH guidelines. The linearity range was found to be 1-25?g/ml with a correlation coefficient of 0.993. The accuracy was found to be within limit. The LOD and LOQ were found to be 1.29 and 4.3 ?g/ml; respectively. The results demonstrated that the method can be conveniently employed for routine quality control analysis of Curcumin in Ayurvedic formulation of Haridrakhand.
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