Bromothymol blue and Bromocresol purple as efficient reagents for the development and validation of visible spectrophotometric assay methods for Felodipine


  • N. Murali Krishna Department of Chemistry, V. R. Siddhartha Engineering College (Autonomous), Vijayawada, Andhra Pradesh, India
  • S. Srinivasa Rao Department of Chemistry, V. R. Siddhartha Engineering College (Autonomous), Vijayawada, Andhra Pradesh, India
  • C. Rambabu Department of Chemistry, Acharya Nagarjuna University, Guntur district, Andhra Pradesh, India


Spectrophotometric analysis, Felodipine, economical methods, validation


Bromothymol blue (BTB) and Bromocresol purple (BCP) are introduced as reagents that can help in developing and validating two simple and low cost visible spectrophotometric methods for the determination of Felodipine drug in pure form as well as in formulations. The new methods are based on attraction between positive charge on the nitrogen of drug molecule and the negative charge of BTB as well as BCP to produce coloured complexes. The coloured complexes exhibited absorption maxima at 420 nm and 415 nm respectively for BTB and BCP. It was found that the results obtained by the proposed methods and the labeled amounts are in good agreement. The relative standard deviations of the proposed methods are found to be 1.557% and 1.807%. Based on all the results, it is concluded that the proposed methods offer low cost, simple, sensitive and rapid process of determining and validating the selected drug using visible spectrophotometry.


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M. J. O. Neil, The Merck Index. Merck Research Laboratories, Whitehouse Station, NJ (2006).

N. C. B. Nyborg, M. J. Mulvany, J. Cardiovascular Pharmacol., 6 (1984) 499-505.

K. Basavaiah, U. Chandrashekar, P. Nage Gowda, J. Serb. Chem. Soc., 7 (2005) 969-978.

Fusun Gedil, Osman Ustun, Okan Atay, Turkish. J. Pharm. Sci., 1 (2004) 65-76.

J. D. Y. Dru, J. Y. K. Hsieh, B. K. Matuszewski, M. R. Dobrinska, J. Chromatogr. B: Biomed. Sci. and Appl., 666 (1995) 259-267.

Margareth Gabrielsson, Kurt-Jurgen Hoffmann, Carl Gunnar Regardh, J. Chromatogr. B: Biomed. Sci. and Appl., 573 (1992) 265-274.

Lars Weidolf, J. Chromatogr. B: Biomed. Sci. and Appl.,343 (1985) 85-97.

K. Basavaiah, U. Chandrashekar, H. C. Prameela, Ind. J. Chem. Techol., 10 (2003) 454-456.

K. S. Nataraj, S. K. Suresh, M. Duza, K. Badrud, Kesinath Teddy, J. Pharm. Res., 4 (2011) 2822.

R. M. Cardoza, P. D. Amin, J. Pharm. Biomed. Anal., 27 (2002) 711-718.

M. I. Rapado, M. C. Garcia-Alvarez-Coque, R. M. Villanueva-Caman, Analyst, 121 (1996) 1677-1682.

J. A. Lopez, V. Martinez, R. M. Alonso, R. M. Jimenez, J. Chromatogr. A, 870 (2000) 105-114.

Hohyun Kim, Hyeongjin Roh, Seung-Bock Yeom, Hee Joo Lee, Sang Beom Han, Chroma-tographia, 58 (2003) 235-240.

V. Sreedevi, P. Rajesh Kumar, T. Rajesh, Int. J. Pharm. Sci. Res., 2 (2011) 65-73.

V. Sreedevi, Putta Rajesh Kumar, Rajesh Thatavarthi, J. S. K. Nagarajan, Der Pharmacia Lettre, 3 (2011) 446- 455.

Yan-yan Li, Yi-Zi YN, Sun Zhi-Zui, X-in LI, Hu Li-Gang, Peng-Fei LI, Zhon GD a- Fang, Chemical Research in Chinese University, 22 (2006) 479-483.

Luis H Migliorança, Rafael E Barrientos-Astigarraga, B. S. Schug, H. H. Blume, Alberto S Pereira, Gilberto De Nucci, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 814 (2005) 217-223.

M. Mathrusri Annapurna, B. Sai Pavan Kumar, S. V. S. Goutam, Indo American Journal of Pharmaceutical Research, 3 (2013) 9277-9285.

Dhale Chaotali, Joshi Suhas, Shete Shubhangi, International journal of pharmacy, 5(2014) 770-772.


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How to Cite

N. Murali Krishna, S. Srinivasa Rao, and C. Rambabu. “Bromothymol Blue and Bromocresol Purple As Efficient Reagents for the Development and Validation of Visible Spectrophotometric Assay Methods for Felodipine ”. International Journal of Pharmaceutics and Drug Analysis, vol. 5, no. 4, Apr. 2017, pp. 108-15,



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