DEVELOPMENT AND VALIDATION OF RATIO SPECTRA DERIVATIVE METHOD FOR THE SIMULTANEOUS ESTIMATION OF BETAHISTINE DIHYDROCHLORIDE AND PROCHLORPERAZINE MALEATE IN TABLET DOSAGE FORM
Keywords:Betahistine Dihydrochloride Prochlorperazine Maleate , Ratio spectra derivative, Tablet dosage form.
The Ratio spectra derivative spectrophotometric method was developed for the simultaneous determination of Betahistine Dihydrochloride (BET) and Prochlorperazine Maleate (PRO) in tablet dosage form. In this method ratio spectra of one drug is taken then they divided by one of the standard concentration of another drug and then converted into first derivative method. The first derivative amplitudes at 216 and 272.40 nm were selected for the determination of PRO and BET respectively. The wavelength interval (Dl) was selected as 8 nm. 0.1 N HCL was used as the solvent. BET shows linearity in range of 4-24?g/ml concentration while PRO shows linearity range 3-18?g/ml concentration. The method was validated statistically and recovery studies were carried out. It was found to be accurate, precise and reproducible. The method was applied to the assay of the drugs in tablet dosage form, which were found in the range of 98.98% and 98.73% of the labeled value for both Betahistine Dihydrochloride and Prochlorperazine maleate. Hence, the method here in described can be suitable for analysis of BET and PRO in quality control of combined pharmaceutical dosage forms.
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