Evaluation of antivenom potential of achyranthes aspera leaves against snake venom

Authors

  • Lata Kothapalli Department of Pharmaceutical Chemistry, Dr. D.Y. Institute of Pharmaceutical Sciences and Research, Maharastra
  • Priyanka R. Gite Department of Pharmaceutical Chemistry, Dr. D.Y. Institute of Pharmaceutical Sciences and Research, Maharastra.
  • Asha Thomas Department of Pharmaceutical Chemistry, Dr. D.Y. Institute of Pharmaceutical Sciences and Research, Maharastra.
  • Rabindra Nanda Department of Pharmaceutical Chemistry, Dr. D.Y. Institute of Pharmaceutical Sciences and Research, Maharastra.
  • Nitin Salvi Haffkine Biopharmaceutical Corporation Ltd

Keywords:

Achyranthes aspera ; russelli’s viper ;Phospholipase A2, Echis carinatus venom HPTLC

Abstract

The antivenom potential of aqueous (AE) and ethanolic extract (EE) of the leaves of Achyranthes aspera plant was evaluated using in vitro assays. Soxhlet extraction of the dried, powdered leaves of Achyranthes aspera was carried out. The neutralization of lethal toxicity induced by D. russelli venom was assessed using mice. Leaf extract effectively neutralized the russeli’s viper venom induced lethality (LD50) of 11 µg with effective dose (ED50) of 0.3mg for AE and 1.5mg for EE. Phospholipase A2 present in snake venom was neutralized at a dose of 0.05mg and 0.06mg of AE and EE leaf extract respectively. Further procoagulant activity was carried out using Echis carinatus venom. The extracts also effectively neutralized the venom induced hemolysis in the concentration range of 50-100µg. HPTLC analysis done for the extracts showed Rf values indicating same constituents in both extracts. The results obtained demonstrate that leaf extract of Achyranthes aspera possess snake venom neutralizing capacity and can be used as an adjuvant for antivenom therapy.

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References

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Published

2016-12-20
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Lata Kothapalli, Priyanka R. Gite, Asha Thomas, Rabindra Nanda, and Nitin Salvi. “Evaluation of Antivenom Potential of Achyranthes Aspera Leaves Against Snake Venom”. International Journal of Pharmaceutics and Drug Analysis, vol. 4, no. 12, Dec. 2016, pp. 505-13, https://ijpda.com/index.php/journal/article/view/259.

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