DEVELOPMENT AND VALIDATION OF RP HPLC MATHOD FOR THE SIMULTANEOUS ESTIMATION OF ATENOLOL, LOSARTAN POTASSIUM AND HYDRO-CHLOROTHIAZIDE IN TABLET FORMULATION
Keywords:Atenolol, Losartan, Hydrochlorthiazide, Method Validation, RP HPLC
A new simple, rapid, precise and accurate assay method was developed for simultaneous estimation of Atenolol, Losartan and Hydrochlorthiazide in pure form and tablet form. The analytes were separated by RP HPLC on a RP-Purosnosphere C18 column (5 µm, 4.6mm* 250 mm).The mobile phase was methanol:water (77:23, v/v) at 1.1 mL/min flow rate satisfactorily resolve the tertiary mixture. The UV detector was operated at 230 nm for the determination of all the drugs. Linearity, accuracy and precision were found to be acceptable over the concentration ranges of 20-120 µg/ml for Atenolol and Losartan while 5-30 µg/ml for Hydrochlorthiazide with a R2 0.9999, 0.9998 and 0.9990 values respectively. The optimized methods proved to be specific, robust and accurate for the quality control of drugs in bulk drug and pharmaceutical formulations.
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