DETERMINATION OF ACITRETIN IN PHARMACEUTICAL FORMULATIONS BY HPLC METHOD

Authors

  • Devika Subramaniyan.G Department of Pharmaceutical Analysis and Quality Assurance Vasavi Institute of Pharmaceutical Sciences, Affiliated to JNTUA, Kadapa-516247, Andhra Pradesh, India.
  • Shaik yasmin Department of Pharmaceutical Analysis and Quality Assurance Vasavi Institute of Pharmaceutical Sciences, Affiliated to JNTUA, Kadapa-516247, Andhra Pradesh, India
  • R.Swathi Department of Pharmaceutical Analysis and Quality Assurance Vasavi Institute of Pharmaceutical Sciences, Affiliated to JNTUA, Kadapa-516247, Andhra Pradesh, India.
  • S.Amatul Azeem Department of Pharmaceutical Analysis and Quality Assurance Vasavi Institute of Pharmaceutical Sciences, Affiliated to JNTUA, Kadapa-516247, Andhra Pradesh, India.
  • Ramesh petchi.R Department of Pharmaceutical Analysis and Quality Assurance Vasavi Institute of Pharmaceutical Sciences, Affiliated to JNTUA, Kadapa-516247, Andhra Pradesh, India
  • M.Kiran kumar Department of Pharmaceutical Analysis and Quality Assurance Vasavi Institute of Pharmaceutical Sciences, Affiliated to JNTUA, Kadapa-516247, Andhra Pradesh, India.
  • M.Purushothaman Department of Pharmaceutical Analysis and Quality Assurance Vasavi Institute of Pharmaceutical Sciences, Affiliated to JNTUA, Kadapa-516247, Andhra Pradesh, India.

Keywords:

Acitretin, HPLC, Validation, ICH

Abstract

Acitretin is in a group of medicines called retinoids. It is used in severe skin disorders like psoriasis and it is official in USP. Psoriasis is a skin disease affecting millions of person’s worldwide. A simple, precise and rapid HPLC method was developed for the estimation of Acitretin in pharmaceutical dosage forms. The method was carried out on a Purospher BDSC18 column(250×4.6mmid, 5µm) column using a mixture of acetic acid Buffer (pH adjusted to 4): methanol: tetrahyrofuran [12:85:3]v/v/v as a mobile phase. The flow rate was 1 ml/min and Ultra-violet detection was carried out at 354 nm. Every part of determination was performed at ambient column temperature. The retention time was 4.313 min for Acitretin. The developed method was validated for parameters like specificity, accuracy, precision, robustness as per International Conference on Harmonization guidelines. Linearity for Acitretin was in the range of 30-180µg/ml and correlation coefficient was found to be 0.999. The percentage recovery was found to be in the limit of 98.9-99.8 %. Statistical analysis of the results has been carried out revealing high accuracy and good precision. Hence this method can be of use and value for the quality control department of pharmaceutical companies manufacturing these formulations without any interference due its sensitivity, simplicity and selectivity.

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References

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Published

2016-03-31
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How to Cite

Devika Subramaniyan.G, Shaik yasmin, R.Swathi, S.Amatul Azeem, Ramesh petchi.R, M.Kiran kumar, and M.Purushothaman. “DETERMINATION OF ACITRETIN IN PHARMACEUTICAL FORMULATIONS BY HPLC METHOD”. International Journal of Pharmaceutics and Drug Analysis, vol. 4, no. 3, Mar. 2016, pp. 147-52, https://ijpda.com/index.php/journal/article/view/207.

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