• NAGAMANI JE Department of Biochemistry, Garden City College of Science and Management studies, Virgo Nagar, Bangalore – 560 049, Karnataka, India.
  • SUKANYA Department of Biochemistry, Garden City College of Science and Management studies, Virgo Nagar, Bangalore – 560 049, Karnataka, India.
  • SAMIM ANSARI Department of Biochemistry, Garden City College of Science and Management studies, Virgo Nagar, Bangalore – 560 049, Karnataka, India.


Cocos nucifera, Mangifera indica, Antioxidant, Antihemolytic, Erythrocytes, Malondialdehyde


Cocos nucifera and Mangifera indica are being exploited largely for its wide therapeutic applications around the world. Present study investigated antioxidant and antihemolytic activities of flower extracts of Cocos nucifera and Mangifera indica. Aqueous, methanol, acetone, diethyl ether and hexane extracts were used for the study. Antioxidant competence of the five extracts was assessed by DPPH and TBARS method. Antihemolytic assay was determined using erythrocytes model and the extent of membrane damage was determined by quantifying Malondialdehyde. Among all the five extracts studied, methanolic extract of Cocos nucifera exhibited significant antioxidant as well as antihemolytic activities.


Download data is not yet available.


Mandal S, Hazra B, Sarkar R, Biswas S, Mandal N. Assessment of the antioxidant and reactive oxygen species scavenging activity of methanolic extract of Caesalphinia crista leaf. Evid Based Complement Alternat Med 2009; 1-11.

Halliwell B, Gulteridge JM. Role of free radicals and catalytic metal ions in human diseases. Methods Enzymol 1990; 105: 105-114

Cheeseman KH, Scater TF. Free radical in medicine. British Medical Bulletin 2003; 49: 479-724

Ghasemi K, Ghasemi Y, Ebrahimzadeh MA., (2009). Antioxidant activity, phenol and flavonoid contents of 13 Citrus species peels and tissues. Pak J Pharm Sci., 22(3): 277-281

Shiva Shankar Reddy CS, Subramanyam MV, Vani R, Asha Devi S., (2007). In vitro models of oxidative stress in rat erythrocytes: effect of antioxidant supplements. Toxicol In Vitro, 21: 1355-1364

Chaudhuri S, Banerjee A, Basu K, Sengupta B,Sengupta PK., (2007). Interaction of flavonoids with red blood cell membrane lipids and proteins: Antioxidant anantihemolytic effects. Int J Biol Macromol., 41: 42-48

Bradford MM. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry 1976; 7: 248-254

Kujala TS, Loponen JM, Klika KD, Pihlaja K. Phenolics and betacyanins in red beetroot: Distribution and effect of cold storage on the content of total phenolics and three individual compounds. J Agri Food Chem 2000; 48: 5388-5342

Woisky Y Salatino. Preparation of water and ethanol extract of Propolis and evaluation of preparation. Bios Biotechnol Biochem 1998; 62: 2230-2232

Sadasivam S, Manickam A. Estimation of dehydroascorbic acid. Biochemical Methods 1997; 184-186

Harborne JB. Phytochemical methods: A guide to modern techniques of analysis. 2nd edition, Chapman and Hall Publishers, New York. 1973; 85-98

Habila I, Bellol A, Dzikwi AA, Musa, Abubakar N. Total phenolics and antioxidant activity of Tridax procumbens Linn. African J Pharmacy and Pharmacology 2010; 4(3): 123-126

Shang-Tzen chang, Jyh-Horng Wu, Sheng-Yang Wang, Pie-Ling Kang, Ning-Sun Yang. Antioxidant activity of extracts from Acacia Confusa bark and heartwood. J Agri Food Chem 2001; 49: 3420-3424

Khanna S, Shiv Prasad HN, Devi K. In vitro antioxidant screening models. Review Pharmacology Chemistry 2004; 38: 180-183

Yen GC, Duh P D. Antioxidant properties of methanolic extracts from peanut hulls. J Am Oil Chem Soc 1993; 70:383-386.

Ebrahimzadeh MA, Nabavi SF, Nabavi SM. Antihemolytic and antioxidant activity of Hibiscus esculentus leaves. Pharmacology 2009; 2: 1097-1105

Hodges DM, DeLong JM, Forney CF, Prange RK. Improving the thiobarbituric acid-reactive-substances assay for esti-mating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 1999; 207: 604-611

Gordon, M. H.; Weng, X. C. Antioxidant Properties of Extracts from Tanshen (Salvia miltiorrhiza Bunge). Food Chem. 1992, 44, 119-122.

Gu, L. W.; Weng, X. C. Antioxidant Activity and Components of Salvia plebeian a Chinese Herb. Food Chem. 2001, 73, 299-305.

Cesquini, M; Torsoni, M.A; Stoppa, G.R; Ogo, S.H. T- BOOH- Induced Oxidative Damage in Sickle Red Blood Cells and the Role of Flavonoids. Biomed. Pharmacother. 2003, 57, 124-129.

Soobrattee MA, Neergheen VS, Luximon Ramma A, Aruoma OI, Bahorun T. Phenolics as potential antioxidant therapeutic agents: Mechanism and actions. Mutation Research 2005; 579: 200-213

Zeng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agri Food Chem 2001; 49: 5165-5170

Niki E. Antioxidants in relation to lipid peroxidation. Chemical physical Lipids. 1982; 44: 227-253.


16 Views | 11 Downloads

How to Cite

NAGAMANI JE, SUKANYA, and SAMIM ANSARI. “CYTOPROTECTIVE ACTIVITY OF COCOS NUCIFERA AND MANGIFERA INDICA FLOWER EXTRACTS ”. International Journal of Pharmaceutics and Drug Analysis, vol. 4, no. 2, Feb. 2016, pp. 79-84,



Research Articles
Share |