In vitro Free Radical Scavenging Activity, Phytochemical Screening and Quantitative Analysis of Passiflora ligularis Seed


  • Ramachandran Azhid Department of Biochemistry, Karpagam University, Coimbatore-641 021, India
  • Suriyamoorthy Priyanga Department of Biochemistry, Karpagam University, Coimbatore-641 021, India
  • Subrhamanian Hemmalakshmi Department of Biochemistry, Karpagam University, Coimbatore-641 021, India
  • Devaki Kanakasabapathy Department of Biochemistry, Karpagam University, Coimbatore-641 021, India


Tenofovir, Emtricitabine, Simultaneous Estimation, Validation, HPLC


The medicinal properties of plants are due to the presence of chemical substances that produce certain definite physiological action on the human body. The qualitative analysis as well as quantification of phytochemicals of a medicinal plant is regarded as fundamental step in any kind of medicinal plant research. Ethanolic extract of Passiflora ligularis seed was screened for secondary metabolites such as flavonoids, tannins and carbohydrates. Total alkaloid, flavonoid, phenol, saponin and tannin contents were also analysed quantitatively. The preliminary phytochemical screening revealed presence of flavonoids, saponins, tannins, phenols and glycosides. Analysis of the free radical scavenging activity of the extract exposed a concentration dependent antiradical activity resulting from the reduction of radicals to non-radical forms. Findings of the study provided support that crude extract of the plant species contains medicinally important bioactive compounds and justify the use of the plant in the traditional medicine for the treatment of different diseases.


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Habila, J.D., Bello, I.A., Dzikwe, A.A., Ladan, Z., Sabiu, M., 2011. Comparative evaluation of phytochemicals, antioxidant and antimicrobial activity of four medicinal plants native to Northen Nigeria. Aust. J. Basic. Appl. Sci. 5, 537-543.

Gulcin I., 2005. The antioxidant and radical scavenging activities of black pepper seeds. Int. J. Food. Sci. Nutr. 56, 491-499.

Rimbach, G., Fuchs, J. Packer, L. Application of nutrigenomics tools to analyze the role of oxidants and antioxidants in gene expression. Taylor and Francis Boca Raton Publishers: 1-12, (2005).

Rajeshwar, Y., Gupta, M, Mazumder, U.K., 2005. Antitumor and in vivo antioxidant status of Mucuna pruriens (Fabaceae) seeds against Ehrlich ascites carcinoma in Swiss albino mice. Iran. J. Pharm. Ther. 4, 46-53.

Ghoshal, S., Tripathi, V.K., Chauhan, S., 1996. Active constituents of Emblica officinalis. Part I, the Chemistry and antioxidative effects of two hydrolysable tannins, emblicanin A and B. Indian. J. Chem. 35, 941-948.

Erkan, N., Ayranci, G., Ayranci, E., 2008. Antioxidant activity of rosemary (Rosmarinus officinalis) extract, Black seed (Nigella sativa) essential oil, carnosic acid, rosmarinic acid and sesamol. Food. Chem. 110, 76-82.

Youwei, Z., Jinlian, Z., Yonghong, P., 2008. A comparative study on the free radical scavenging activities of some fresh flowers in southern China. LWT. Food. Sci. Technol. 41, 1586-1591.

Saha, M.R., Hasan, S.M.R., Akter, R., Hossain, M.M., Alam, M.S., Alam, M.A., 2008. In vitro free radical scavenging activity of methanol extract of the leaves of Mimusops elengi Linn. Bangl. J. Vet. Med. 6, 197-202.

Krishnaiah, D., Sarbatly, R., Bono, A., 2007. Phytochemical antioxidants for health and medicine – A move towards nature. Biotechnol. Mol. Biol. Rev. 1, 97.

Moran, J.F., Klucas, R.V., Grayer, R.J., Abian, J., Becana, M., 1997. Complexes of iron with phenolic compounds from soybean nodules and other legume tissues: prooxidant and antioxidant properties. Free. Radical. Bio. Med. 22, 861–870.

Hertog, M.G.L., Feskens, E.J.M., Kromhout, D., Hollman, P.C.H., Katan, M.B., 1993. Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen elderly study. Lancet. 342, 1007–1011.

Huxley, R.R., Neil, H.A., 2003. The relation between dietary flavonol intake and coronary heart disease mortality: ametaanalysis of prospective cohort studies. Eur. J. Clin. Nutr. 57, 904-908.

Andrade, D., Gil, C., Breitenfeld, L., Domingues, F., Duarte, A., 2009. Bioactive extracts from Cistus ladanifer and Arbutus unedo L. Indian. Crop. Prod. 30, 165-167.

Alim, A., Goze, I., Goze, H., Tepe, B., 2009. In vitro antimicrobial and antiviral activities of the essential oil and various extracts of Salvia cedronella Boiss. J. Med. Plants. Res. 3, 413-419.

Ajila, C.M., Rao, J., Prasada Rao, U.J.S., 2010. Characterization of bioactive compounds from raw and ripe Mangifera indica L. peel extracts. Food. Chem. Toxicol. 48, 3406-3411.

Pratt, D.E., Hudson, B.J.F. Natural antioxidants not exploited commercially. First edition. Food Antioxidants: 1990.

Rice Evans, C.A., Miller, N.J., Paganga, G., 1996. Structure – antioxidant activity relationships of flavonoids and phenolic acids. Free. Radical. Biol. Med. 20, 933–956.

Dhawan, K., Dhawan, D., Sharma, A., 2004. Passiflora: a review update. J. Ethnopharmacol. 94, 1-23.

Konczak, I., Roulle, P., 2001. Nutritional properties of commercially grown native Australian fruits: Lipophilic antioxidants and minerals. Food. Res. Int. 44, 2339–2344.

Krishnaiah, D., Sarbatly, R., Nithyanandam, R., 2011. A review of antioxidant potential of medicinal plant species. Food. Bioprod. Process. 89, 217–233.

Trease GE, Evans WC. Pharmacognosy 4th Edition, Saunders: 1996.

Harborne J.B. Phytochemical Methods. Chapman and Hall Ltd: 1973.

Blois M.S., 2000. Antioxidant determinations by the use of stable free radical. Nature. 81, 1199-2000.

Re, R., Pelligrini, N., Proteggeenate, M., Yang, C., Evans, R., 1999. Antioxidants activity of applying an improved ABTS radical cation decolorisation assay. Free. Rad. Biol. Med. 26, 1231-1237.

Klein, S.M., Cohen, G., Cederbaum, A.I., 1991. Production of formaldehyde during metabolism of dimethyl sulphoxide by hydroxyl radical generating system. Biochem. 20, 6006-6012.

Ruch, R.J., Cheng, S.J., Klaunig, J.E., Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis.10, 1003-1008.

Green, L.C., Wagner, D.A., Glogowski, J., Skipper, P.L., Wishnok, J.S., Tannenbaum, S.R., 1982. Analysis of nitrate, nitrite and nitrate in biological fluids. Anal. Biochem. 126, 131–138.

Liu, F., Ooi, V.E.C., Chang, S.T., 1997. Free radical scavenging activity of mushroom polysaccarides extract. Life. Sci. 60, 763-771.

Dinis, T.C.P., Madeira, V.M.C., Almeida, L.M., 1994. Action of phenolic derivatives (Acetoaminophen, Salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch. Biochem. Biophys. 315, 161-169.

Oyaizu, M., 1986. Studies on product of browning reaction prepared from glucose amine. Jpn. J. Nutr. 7, 307-315.

Benzie, I.F.F., Strain, J.J., 1996. Ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal. Biochem. 239, 70-76.

Malik, E.P., Singh, M.B. Plant Enzymology and Hittoenzymology. 1st Edition. Kalyani Publishers: 1980.

Boham, B.A., Kocipai-Abyazan, R., 1974. Flavonoids and condensed tannins from leaves of Hawaiian Vaccinium vaticulatum and Vaccinium calycinium. Pacific. Sci. 458448-458463.

Obdoni, B.O., Ochukom, P.O., 2001. Phytochemical studies and comparative efficacy of the crude extracts of some Homostatic plants in Edo and Delta States of Nigeria Global. J. Pure. Appl. Sci. 8, 203-208.

Chanwitheesuk, A., Aphiwat, T., Rakariyatham, N., 2005. Screening of antioxidant activity and antioxidant compounds of some edible plants of Thailand. Food. Chem. 92, 491–497.

Ozyurt, D., Ozturk, B.D., Apak, R. Determination of total flavonoid content of Urtica dioica L. by a new method. Proceedings book. Thrkey: Adnan menderes University, 4th AACD Congress: 30, (2004).

Heim, K.E., Tagliaferro, A.R., Bobilya, D.J., 2002. Flavonoid antioxidants: Chemistry, metabolism and structure-activity relationships. J. Nutr. Biochem. 13, 572-584.

Dipankar, C., Nikhil, B.G., Rhitajit, S., Nripendranath, M., 2012. Phytochemical analysis and evaluation of antioxidant and free radical scavenging activity of Withania somnifera root. Asian. J. Pharm. Clin. Res. 5, 193-199.

Kessler, M., Ubeaud, G., Jung, L., Anti- and pro-oxidant activity of rutin and quercetin derivatives. J. Pharm. Pharmacol. 55, 131-142.

Sangeetha, M.S., Priyanga, S., Hemmalakshmi, S., Devaki, K., In vivo antidiabetic potential of Cyclea peltata in strepto-zotocin?induced?diabetic rats. Asian. J. Pharmaceut. Clin. Res. 8, 103-108.

Priyanga, S., Mary, M.R.F., Hemmalakshmi, S., Devaki, K., 2014. Anti hyperlipidemic effect of aqueous extract of Aegle marmelos and Camellia sinensis in oil fed hyperlipidemic rats. Int. J. Pharm. Pharm. Sci. 6, 338-341.

Priyanga, S., Hemmalakshmi, S., Sowmya, S., Vidya, B., Chella Perumal, P., Gopalakrishnan, V.K., Devaki, K., 2015. In vitro enzyme inhibitory evaluation and free radical scavenging potential of ethanolic leaf extract of Macrotyloma uniflorum (L.). Int. J. Cur. Pharmaceut. Rev. Res. 6, 169-177.

Joseph, A., Hemmalakshmi, S., Priyanga, S., Devaki, K., 2014. Phytochemical screening, GC-MS analysis and enzyme inhibitory activity of Passiflora Foetida L. Indo. Amer. J. Pharmaceut. Res. 4, 3526-3534.

Chang, L.W., Yen, W.J., Huang, S.C., Duh, P.D., 2002. Antioxidant activity of sesame coat. Food. Chem. 78, 347-354.

Mondal, S.K., Chakraborty, G., Gupta, M., Mazumder, U.K., 2006. In vitro antioxidant activity of Diospyros malabarica Kostel bark. Indian. J. Exp. Biol. 44, 39-44.

Rajeshwar, Y., Gupta, M., Mazumder, U.K., 2005. Antitumor and in vivo antioxidant status of Mucuna pruriens (Fabaceae) seeds against Ehrlich ascites carcinoma in Swiss albino mice. Iranian. J. Pharmaceut. Ther. 4, 46-53.

Sanmugapriya, K., Saravana, P.S., Payal, H., Peer Mohammed, S., Binnie, W., 2011. Antioxidant activity, total phenolic and flavanoid contents of Artocarpus heterophyllus and Manilkara zapota seeds and its reduction potential. Int. J. Pharm. Pharmaceut. Sci. 3, 256-260.

Chew, Y.L., Goh, J.K., Lim, Y.Y., 2009. Assessment of in vitro antioxidant capacity and polyphenolic composition of selected medicinal herbs from Leguminosae family in Peninsular Malaysia. Food. Chem. 116, 13-18.

Gutteridgde, M.C., 1995. Free radicals in disease processes: A complication of cause and consequence. Free. Radical. Res. 19, 141-158.

Chidambaram, U., Pachamuthu, V., Natarajan, S., Elango, B., Suriyanarayanan., Ramkumar, K.M., 2013. In vitro evaluation of free radical scavenging activity of Codariocalyx motorius root extract. Asian. Pac. J. Trop. Med., 188-194.

Nabavi, S.M., Ebrahimzadeh, M.A., Nabavi, S.F., Jafari, M., 2008. Free radical scavenging activity and antioxidant capacity of Eryngium caucasicum, trautv and Floripia Subpinnala. Pharmacologyonline. 3, 19-25.

Dharani, B., Sumathi, S., Sivaprabha, J., Padma, P.R., 2011. In vitro antioxidant potential of Prosopis cineraria leaves. J. Nat. Prod. Plant. Resources. 1, 26-32.

Halliwell B, Gutteridge J.M.C. Free radicals in biology and medicine. 3rd edition, oxford university press: 1999.

Chandra Mohan, S., Balamurugan, V., Thiripura Salini, S., Rekha, R., 2012. Metal ion chelating activity and hydrogen peroxide scavenging activity of medicinal plant Kalanchoe pinnat. J. Chem. Pharmaceut. Res. 4, 197-202.

Anjali, S., Sheetal, S., 2013. Phytochemical analysis and free radical scavenging potential of herbal and medicinal plant extracts. J Pharmacol Phyt. 2, 22-29.

Luximon-Ramma, A., Bahorun, T., Soobrattee, M.A., Aruoma, O.I., 2002. Antioxidant activities of phenolic, proanthocyanidin, and flavonoid components in extracts of Cassia fistula. J. Agric. Food. Chem. 50, 5042-5047.

Kalaiselvi, M., Ravikumar, G., Gomathi, D., Uma, C., 2012. In vitro free radical scavenging activity of Ananus comosus (l.) Merrill peel. Int J Pharm Pharmaceut Sci. 4, 604-609.

Priyanga, S., Hemmalakshmi, S., Devaki, K., 2014. Comparative phytochemical investigation of leaf, stem, flower and seed extracts of Macrotyloma Uniflorum L.. Indo. Amer. J. Pharmaceut. Res. 4, 5415-5420.

Mbaebie, B.O., Edeoga, H.O., Afolayan, A.J., 2012. Phytochemical analysis and antioxidants activities of aqueous whole plant bark extract of Schotia latifolia Jacq. Asian. Pac. J. Trop. Biomed. 2, 118-124.

Priyanga, S., Hemmalakshmi, S., Sowmya, S., Vidya, B., Chella Perumal, P., Gopalakrishnan, V.K., Devaki, K., 2015. Quantitative evaluation and in vitro free radical scavenging ability of ethanolic stem extract of Macrotyloma uniflorum L. Der. Pharmacia. Lettre.7, 225-233.


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Ramachandran Azhid, Suriyamoorthy Priyanga, Subrhamanian Hemmalakshmi, and Devaki Kanakasabapathy. “In Vitro Free Radical Scavenging Activity, Phytochemical Screening and Quantitative Analysis of Passiflora Ligularis Seed”. International Journal of Pharmaceutics and Drug Analysis, vol. 4, no. 2, Feb. 2016, pp. 49-57,



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