DEVELOPMENT AND VALIDATION OF UV SPECTROPHO-TOMETRIC METHOD FOR ESTIMATION OF SAXAGLIPTIN IN BULK AND PHARMACEUTICAL DOSAGE FORM
Keywords:Saxagliptin (onglyza); Ultraviolet spectrophotometry; Zero order spectrum; First order derivative & spectroscopy
Three simple, precise and economical UV spectrophotometric methods have been developed for the estimation of Saxagliptin in bulk and pharmaceutical formulations. Saxagliptin is an antidiabetic drug belonging the chemical class is dipeptidyl peptidase-4 enzyme (DPP-4) inhibitor. Saxagliptin has absorbance maxima at 211 nm in zero order spectrum method (Method A), and in the first order derivative spectra, showed sharp peak at 204 nm when n = 1 (Method B). The drug followed the Beer- Lambert’s law in the concentration range of 5-50 µg/ml in all two methods. Results of the analysis, validated statistically and by recovery studies were found to be satisfactory.
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