DEVELOPMENT AND VALIDATION OF VISIBLE SPECTROMETRIC METHOD FOR DETERMINATION OF NICOTINE IN GUM TABLET
Keywords:Nicotine, Folin-Ciocalteu, Chromogen, Spectrophotometric
A simple, sensitive, accurate and rapid Spectrophotometric method has been developed for the estimation of nicotine
using Folin-Ciocalteu reagent in presence of 20% sodium carbonate solution. The blue color chromogen formed is
measured at wavelength of maximum absorption 760 nm, respectively against reagent blank. The chromogen obeyed
linearity over 3-14 ?g/ml for nicotine. The results of analysis have been validated statistically and by recovery studies.
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