METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ATORVASTATIN, FENOFIBRATE AND FOLIC ACID IN PHARMACEUTICAL DOSAGE FORM
Keywords:RP-HPLC, Atorvastatin, Fenofibrate and Folic acid, Validation
A simple, rapid, accurate and precise isocratic reverse phase high performance liquid chromatography (RP-HPLC) method
has been developed for simultaneous estimation of atorvastatin, fenofibrate and folic acid in combined dosage form by
using C18 column. Mobile phase consisting of a 40 volumes of mixed phosphate buffer (pH 6.0) : 60 volumes of acetonitrile
and detection was carried out at 235 nm and the retention times of atorvastatin, fenofibrate and folic acid were found to be
4.407, 3.393and 7.450 min respectively. The developed method was validated as per ICH guideline for specificity, linearity,
accuracy, and precision and system suitability. The new RP-HPLC method was successfully applied to marketed
formulation without any interference from excipients.
How to Cite
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright © Author(s) retain the copyright of this article.