A STABILITY-INDICATING HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF COBICISTAT

Abstract

A new reverse phase – high performance liquid chromatography (RP-HPLC) have been developed and validated for the
estimation of Cobicistat in bulk drug and pharmaceutical dosage form. The developed method is rapid, accurate, precise,
simple and economical. The separation was carried out using column hypersil BDS C-18 (150mm x 4.6mm 5? particle size)
in isocratic mode, with mobile phase containing water: Acetonitrile (90:10). The flow rate 1.0 ml/min effluents are
monitored at 240 nm. Chromatogram showed peak at a retention time of 4.09 min for Cobicistat. The method is validated
according to ICH guidelines system suitability, linearity, precision, accuracy, specificity, ruggedness, robustness, LOD and
LOQ. The calibration plot showed good linear relationship with r2 = 0.999 in the concentration range of 7.5 to 45 ?g/ml for
Cobicistat. The LOD and LOQ were found to be 0.1472?g/ml and 0.4461 ?g/ml. accuracy was found to be 99.61 %.
Cobicistat also undergoes degradation in acids, alkalies, thermal and photolytic conditions. The method is specific for the
estimation of Cobicistat in Cobicistat tablets.